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Showing content with the highest reputation since 09/15/2018 in all areas

  1. 5 points
    Raiden

    Night Owls X: TCO's Up In Smoke Tour

    Were you getting crack? Or getting your dick sucked? Or sucking dick for crack?
  2. 4 points
    Raiden

    Karlsson to San Jose.

    I've pinned the thread. It makes me too happy. It will make me happy always.
  3. 4 points
    HBCYG

    Night Owls X: TCO's Up In Smoke Tour

    I could only be so lucky.
  4. 4 points
    Plot twist: he’s the local crackhead and he was doing the in my feelings challenge
  5. 4 points
    TuckerIntensity

    Zetterberg is done

    Z was a beast. Love that guy.
  6. 4 points
    §andman

    Karlsson to San Jose.

    Florida is light years ahead of Ottawa and Montreal. They'll be right in the middle battling it out.
  7. 3 points
    Danny

    Night Owls X: TCO's Up In Smoke Tour

    I got you bro, seems easy/ (a,b) Representative immunofluorescence images of MEFs with indicated genotypes subjected to 24 h serum starvation. Cells were stained with anti-PC1 (E4, Supplementary Fig. 5) and analysed by confocal microscopy (a), or stained with anti-PC2 and analysed by epifluorescence microscopy (b). Arl13b or acetylated (Ac)-tubulin was used as a ciliary marker. Arrows indicate cilia. (c) The bar diagram shows the quantification of PC1 or PC2 ciliary localization in MEFs with various genotypes with numbers of cells analysed (n) indicated; ***P<0.001. (d) Exogenously expressed PC1 traffics to cilia (arrow) and rescues ciliary localization of PC2 in Pkd1−/− MEFs. Exogenous expression of GFP served as a negative control (Supplementary Fig. 1). (e) Confocal images of CD cells (upper panel) and CD cells expressing Pkd2 shRNA (lower panel). Cells were stained with antibodies against Arl13b (blue) and PC1(E4) (red). The bar diagram below shows the quantification of PC1 ciliary localization in CD and cells with Pkd2 knockdown performed from four independent experiments with numbers of cells analysed (n) indicated; ***P<0.001. Western blot analysis shows that Pkd2 shRNA reduced endogenous PC2 level by ~90%. (f) Confocal images of CD cells (upper panel) and cells expressing Pkd1 shRNA, stained with antibodies as indicated. Four different Pkd1shRNAs had similar results. The bar diagram shows the quantification of PC2 ciliary localization between CD and cells with Pkd1 knockdown from four independent experiments; ***P<0.001. Western blot analysis shows that Pkd1 shRNA reduced endogenous PC1 level by ~90%. The white line in the XY scan in (e,f) indicates the path of the XZ scan. (g) Confocal images of PC2 ciliary localization in collecting duct at P28 in Pkd1cko/cko:tamoxifen-Cre mouse kidney33, in which Pkd1 was not inactivated (left panel, normal tubule) or inactivated by tamoxifen injection on P10 (right panel, cystic tubule). PC2 was detected in 21 of 21 cilia (red) in normal kidneys. Cilia were marked by acetylated tubulin (green). Note that PC2 was not detectable in 18 of 18 cilia of cysts analysed. Scale bar, 10 μm. https://www.nature.com/articles/ncomms6482/figures/1 (basically, throw out pc3)
  8. 3 points
    Sypher_04

    Tapatalk

    I firmly believe Tapatalk to be hot garbage if I'm being honest
  9. 3 points
    Danny

    Night Owls X: TCO's Up In Smoke Tour

    ROFL So, cell phone says you missed a call (from our son) Wife dials back. music is blaring! she screams,...turn it down! I use my peripheral and see a stage and said,..He is at the Metallica concert. lmao.."turn it down"
  10. 3 points
    tbnl

    Zetterberg is done

  11. 3 points
    Raiden

    Zetterberg is done

    Him and Dats are the poster child for defence first, offence second. They were both unreal. It's a shame he didn't go out on his terms though
  12. 3 points
    §andman

    Zetterberg is done

    Solid player and classy. Must be because he stopped Crosby effectively.
  13. 2 points
    Too much pron.
  14. 2 points
    Oh, so he's doing side quests?
  15. 2 points
    TuckerIntensity

    Zetterberg is done

    He's the reason Crosby didn't win the Conn Smythe when he should have. It was unfortunate. Almost as unfortunate when Crosby won the one he shouldn't have. I know Babcock wants Matthews to be more like Z, and I don't blame him. Great leadership, 200 foot player. He's a good guy to model those parts of his game after.
  16. 2 points
    Morrison7

    John Tavares Is A Leaf

    Who needs Tavares anyways when you can have a bunch of 4th liners with playoff experience? 😐
  17. 2 points
    Sypher_04

    Currently Playing?

    I agree on most of this. I started this game and was just consumed by it for 2 days. Still playing gonna 100% it but I basically played the story non stop. The only part I disagree with is the low bar comment. This game is best imo but the arkham games are pretty terrific
  18. 2 points
    majingir

    Your 2018/2019 Toronto Maple Leafs

    lol, Marner looks like a kid going on a fieldtrip with his grandfather (Rick) being a parent volunteer
  19. 2 points
    Raiden

    Night Owls X: TCO's Up In Smoke Tour

    So TCO knocked him out to save everyone?
  20. 2 points
    Armster

    The Rap and Hip-Hop thread, Resurrected

    Fuck. We needed this Eminem and didn't even realize it.
  21. 2 points
    Raiden

    The Rap and Hip-Hop thread, Resurrected

    Iggy wouldn't know strong bars if it hit her in the face
  22. 2 points
  23. 1 point
    Mantis Toboggan

    Currently Playing?

    rofl "Mister Negative"
  24. 1 point
    No reason really just thought it was a one season thing. Warms my heart to hear otherwise.
  25. 1 point
    TuckerIntensity

    Karlsson to San Jose.

    Formenton will be playing.
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